Jayapriya et al

Siddha System is one of the oldest system of medicine in India. It includes 32 internal medicine and 32 external medicine , totally 64 types of medicine . Vedikara silasathu parpam is one of the internal medicine mentioned in the literature “ Kannusaamiyam parambarai vaithiyam ” which is indicated for Neerkattu. Siddhars had classified the diseases into 4448 types. Yugi had classified urinary disorder into two, Neerinai arukkal noigalandNeerinai perukkal noigal , Neerkattu comes under Neerinai arukkal noikal.

Anuria or anuresis means suppression of urine. It may result from blocking of tubules of the kidney, gross obstruction to the urinary tract or rapid fall of blood pressure. The aim of the study was to evaluate the biochemical analysis of the drug Vedikara silasathu parpamand it indicates the presence of Calcium, Sulphate, Chloride, Carbonates And Ferrous Iron which revealed the enhancement of therapeutic action. Drug with Proper scientific evidence will be beneficial for the mankind in future.

Keywords : Neerkattu, Biochemical analysis, Vedikara silasathu parpam , Siddha medicine

Nowadays the scientific world is turning around to the traditional system of medicine due to their indigenous specificity. Siddha medicine is a system of traditional medicine originating in ancient Tamilnadu in South India. The concept of siddha medicine is based on 96 thathuvas. This system believes that all objects in the universe including human body are composed of basic elements namely earth, fire, water, air and sky. According to siddha medicine system, diet and lifestyle play a major role in health and in curing diseases. Yugi,one of the ancient siddhar had classified urinary disorder into two , Neerinai arukkal noigalandNeerinai perukkal noigal. Neerkattucomes under neerinai arukkal noigal and it is corelated with Anuria. In India approximately 6.4 per 1000 admission patients suffer from anuria and atleast 1/1000 of Indian population needs hospitalization due to this disease. Our Siddhars used many numbers of herbs, animal products, metals and minerals as medicines. I selected mineral drug “vedikara silasathu parpam” which is indicated for Neerkattu.

100 mg of the powdered drug was weighed accurately and placed in a 250 ml clean beaker and added the few drops of conc. Hydrochloric acid and evaporated it well. After evaporation cooled the content and added the few drops of conc. Nitric acid and evaporated it well. After cooling the content add 20ml of distilled water and dissolved it well. Then it is transferred to 100ml volumetric flask and made up to 100ml with distilled water mix well. Filter it. Then it is taken for analysis.

S. No	Tamil Name	English Name	Chemical name	Quantity
1.	Vengaram	Borax	Sodium biborate	2 palam ( 70 gms )
2.	Karpoora silasathu	Gypsum	Calcium sulfate dihydrate	2 palam ( 70 gms )
3.	Vediyuppu	Salt Petre	Potassium nitrate	2 palam ( 70 gms )
4.	Karsunnambu	Limestone	Calcium carbonate	Required quantity

The required drugs were purchased from a reputed country shop at Nagercoil, The identification of the mineral drug is authenticated by the authorities, Department of PG Gunapadam, Palayamkottai.

All the ingredients of the formulation were purified as mentioned in siddha classical literature.

Equal quantity of purified vediyuppu, venkaramand silasathu were grounded together in kalvam for 3 samam ( 9 hours ) / day with lime stone water for 2 days, it is then made into villai and it is sundried. Then these disc were kept in a shallow earthen pan and covered with identical pan inverted over it and edges were sealed with 7 clay smeared cloth ribbon and dried. Then it is subjected to pudam process with 20-25 cow dung cakes. After cooled down , it is finely powdered in a mortar and stored in a air tight container.

Screening the drug “ Vedikara silasathu parpam ”to analyze the biochemical properties present.

The chemicals used for this study were obtained from Department of Biochemistry , Government siddha medical college, palayamkottai.

S.No.	EXPERIMENT	OBSERVATION	INFERENCE
01	TEST FOR CALCIUM 2 ml of the above prepared extract is taken in a clean test tube. To this add 2 ml of 4% Ammonium oxalate solution.	A white precipitate is formed	Indicates the presence of calcium
02	TEST FOR SULPHATE 2 ml of the extract is added to 5% Barium chloride solution.	A white precipitate is formed	Indicates the presence of sulphate
03	TEST FOR CHLORIDE The extract is treated with silver nitrate solution.	A white precipitate is formed	Indicates the presence of chloride
04	TEST FOR CARBONATE The substance is treated with concentrated HCl.	Brisk effervessence is formed	Indicates the presence of carbonate
05	TEST FOR STARCH The extract is added with weak iodine solution.	No blue colour is formed	Absence of starch
06	TEST FOR FERRIC IRON The extract is acidified with Glacial acetic acid and potassium ferro cyanide.	No blue colour is formed	Absence of ferric iron
07	TEST FOR FERROUS IRON The extract is treated with concentrated Nitric acid and Ammonium thiocyanate solution.	Blood red colour is formed	Indicates the presence of ferrous iron
08	TEST FOR PHOSPHATE The extract is treated with Ammonium Molybdate and concentrated nitric acid.	No yellow precipitate is formed	Absence of phosphate
09	TEST FOR ALBUMIN The extract is treated with Esbach’s reagent.	No yellow precipitate is formed	Absence of Albumin
10	TEST FOR TANNIC ACID The extract is treated with ferric chloride.	No blue black precipitate is formed	Absence of Tannic acid
11	TEST FOR UNSATURATION Potassium permanganate solution is added to the extract.	It does not get decolourised	Absence of Unsaturated compound
12	TEST FOR THE REDUCING SUGAR 5 ml of Benedict’s qualitative solution is taken in a test tube and allowed to boil for 2 minutes and add 8-10 drops of the extract and again boil it for 2 minutes.	No colour change occurs	Absence of Reducing sugar
13	TEST FOR AMINO ACID One or two drops of the extract is placed on a filter paper and dried well. After drying, 1% Ninhydrin is sprayed over the same and dried it well.	No violet colour is fromed	Absence of Amino acid
14	TEST FOR ZINC The extract is treated with Potassium Ferro cyanide.	No white precipitate is formed	Absence of Zinc

The bio chemical analysis of the trial drug Vedikara silasathu parpam was tabulated in table 2.

From the above inference it is obvious that why it is indicated for Neerkattu , may be the presence of Calcium, Sulphate, Chloride, Carbonates And Ferrous Iron enhances the activity.

Vedikara silasathu parpam is a mineral drug formulation mentioned in siddha literature and it is indicated for Neerkattu( anuria ). The drug is screened for bio chemical analysis. Further comprehensive physicochemical analysis , heavy metal analysis and animal study are needed to evaluate its potency, safety and efficacy.

1. Kannusaamy, parambarai vaithiyam, B.Rathina nayakar and sons ; 2 ^nd edition 2015; pg no 374, 375.

2. Kuppusamy muthaliyar.K.N, Siddha maruthuvam (pothu), Department of Indian medicine and homeopathy ; 8 ^th edition 2016

3. Thiyagarajan.R L.I.M, Gunapadam thathu – jeeva vaguppu, Indian Medicine and Homeopathy, 8 ^th edition 2013