Screening of minimal inhibitory & fungicidal concentration (MIC & MFC) Of Purified Thurusu ( Copper Sulphate)
Thurusu or Crude copper sulphate(CuSo4) or is used as a raw drug in Siddha system of Medicine, it is available in nature and prepared synthetically by boiling Copper with Sulfuric acid. Chemically it exists in two forms hydrated form copper sulphate pentahydrate, it is blue in colour and anhydrous form copper sulphate ,it is pale green or grey white in colour. It is used after purification as a single drug or one of the ingredients in some metallo mineral formulations, it is widely used for external applications and internal formulations it has astringent, emetic, tonic, caustic and antiseptic actions, thurusu is normally used to heal ulcers, ulcers in male genital organ, eye diseases, tiridhodam etc .
Hence in this pretext the fungicidal concentration of purified thurusu is validated by Minimal inhibitory concentration (MIC) and Minimal fungicidal concentration (MFC) methods.The MFC test allows determination of the minimum concentration of an agent necessary to achieve a fungicidal effect. It is worth noting, however, that the duration of time the antimicrobial is in contact with the test organism is quite long for this method, on the order of 18 hours. Thus, the test truly does determine the minimum concentration needed to kill the test organism, since all other parameters are conducive to biocidal effect. The MFC test can be a good and relatively inexpensive tool to rank a great number of antimicrobial agents by potency, for screening purposes. The MFC test can be used to evaluate formulation problems wherein the formulator suspects that the active ingredient is being “bound up” by other ingredients. The theory is that the MFC will be worse for a formula that has a portion of its active ingredient chemically combined with other ingredients, thus not available to kill microorganisms in the suspension.
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