Screening of minimal inhibitory & fungicidal concentration (MIC & MFC) Of Purified Thurusu ( Copper Sulphate)

  • Ethel Shiny S Associate Professor & HOD, Department of Gunapadam - Marunthiyal , Santhigiri Siddha Medical College, Thiruvananthapuram, Kerala.
  • Bharath Christian Assistant Professor, Department of Maruthuvam , Santhigiri Siddha Medical College, Thiruvananthapuram, Kerala.
  • Gomathi P Assistant Professor, Department of Varmam, Pura Maruthuvam and Sirappu Maruthuvam , Thiruvananthapuram, Kerala.
Keywords: Thurusu, MIC and MFC , Pre- clinical studies, Anti-fungal


Thurusu or  Crude copper sulphate(CuSo4) or is used as a raw drug in Siddha system of Medicine, it is available in nature and prepared synthetically by boiling Copper with Sulfuric acid. Chemically it exists in two forms hydrated form copper sulphate pentahydrate, it is blue in colour and anhydrous form copper sulphate ,it is pale green or grey white in colour. It is used after purification as a single drug or one of the ingredients in some metallo mineral formulations, it is widely used for external applications and internal formulations it has astringent, emetic, tonic, caustic and antiseptic actions, thurusu is normally used to heal ulcers, ulcers in male genital organ, eye diseases, tiridhodam etc .

            Hence in this pretext the fungicidal  concentration of purified thurusu is validated by Minimal inhibitory concentration (MIC) and Minimal fungicidal concentration (MFC) methods.The MFC test allows determination of the minimum concentration of an agent necessary to achieve a  fungicidal effect. It is worth noting, however, that the duration of time the antimicrobial is in contact with the test organism is quite long for this method, on the order of 18 hours. Thus, the test truly does determine the minimum concentration needed to kill the test organism, since all other parameters are conducive to biocidal effect. The MFC test can be a good and relatively inexpensive tool to rank a great number of antimicrobial agents by potency, for screening purposes. The MFC test can be used to evaluate formulation problems wherein the formulator suspects that the active ingredient is being “bound up” by other ingredients. The theory is that the MFC will be worse for a formula that has a portion of its active ingredient chemically combined with other ingredients, thus not available to kill microorganisms in the suspension.


1. Drugs and Cosmetics act 1940 with Drugs and Cosmetics Rules, (Ministry of Health and Family Welfare, Government of India, New Delhi) (2016) p. 317.
2. Subbarayappa BV. Chemical practices and alchemy. In: Bose DM, Sen SN, Subbarayappa BV, editors. A Concise History of Science in India. New Delhi: Indian National Science Academy.
3. Lane, T. W., and F. M. Morel. 2000. A biological function for cadmium in marine diatoms, Proc Natl Acad Sci U S A, 97: 4627-31.
4. Sundararajan SR, Subramanian S. Qualitative assessment of Kayathirumeni- a Siddha medicated oil as a remedy for arthritic pain.
5. Sen CK, Khanna S, Venojarvi M, Trikha P, Ellison EC, Hunt TK, Roy S. Copper-induced vascular endothelial growth factor expression and wound healing. Am J Physiol Heart Circ Physiol. 2002 May; 282(5):H1821-7. doi: 10.1152/ajpheart. 01015.2001. PMID: 11959648.
6. Pharmocpoeial Laboratory for Indian Medicine (PLIM) Guideline for standardization and evaluation of Indian medicine which include drugs of Ayurveda, Unani and Siddha systems. Department of AYUSH Ministry of Health & Family Welfare, Govt. of India.
7. Dhanalakshmi R, Sureka A, Madhavan R. Standardization and Comparative Analysis of Metalloid Based Novel Siddha Preparation Thurusu. Int. J. Curr. Res. Med. Sci. 2018; 4 (5): 24-9.
8. Subbarayappa BV. Siddha medicine: an overview. The Lancet. 1997 Dec 20; 350(9094): 1841-4.R.
9. Thiagarajan, Gunapaadam- Thathu Jeeva Vaguppu, Department of Indian Medicine and Homeopathy Publishers, B.I.M., 4th edition, 2004.
10. Arunadevi R, Susila R, Murugammal S, Divya S. Preparation and standardization of Mathan Tailam: A classical Siddha formulation for diabetic ulcerative wound healing. Journal of Ayurveda and integrative medicine. 2020 Jan 1; 11(1):10-5.
11. Sharma PV. Siddha medicine. In: Sharma PV, editor. History of Medicine in India. New Delhi: The Indian National Science Academy; 1992. pp. 445–50.
12. Arunvanan, M. & Ramkumar, G.G. & Kumar, Sasi & Subramanian, A. & Mathiaas, M.. (2012). Anti- microbial activity of "Thurusu thaivelai karukku" in the management of respiratory diseases. 4. 10-11.
How to Cite
Ethel Shiny S, Bharath Christian, Gomathi P. Screening of minimal inhibitory & fungicidal concentration (MIC & MFC) Of Purified Thurusu ( Copper Sulphate). J Res Bio Med Sci [Internet]. 2023Mar.26 [cited 2023May30];5(1):136-40. Available from: