In-vitro pharmacological screening of Parangipattai Kudineer chooranam - a herbal formulation used for Kalanjagapadai ( Psoriasis )

  • Archana K
  • Mahadevan MV
  • Mahalakshmi V
  • Muthukumar NJ
DOI: https://doi.org/10.124583/jrbms.v2i4.42
Keywords: Siddha Medicine, HaCaT cell line, Protein denaturation assay, Parangipattai Kudineer chooranam (PKC),

Abstract

Background:Traditional Siddha medicines have been used in the management of skin diseases (i.e chronic diseases) for a long time. This current research was intricated to evaluate in vitro preclinical pharmacological studies for the specific actions related to Kalanjagapadai ( Psoriasis ), skin disorders.

Methodology: The properties of PKC vizanti-inflammatory, Immunomodulatory and Anti-Psoriaticof parangipattai kudineer chooranam was investigated by protein denaturation assays, RAW macrophage cell line assays and HaCat cell lines through in vitro model.The study was started after knowing the solubility profile of Parangipattai Kudineer chooranam and having good soluble in water hence used as solvent. The dose range of 10, 50, 100, 150, 200 and 250 μg/ml were used for respective activities depend on the requirements. 

Results: The test drug Parangipattai kudineer chooranam exhibited strong anti-inflammatory ability in vitro compared with standard diclofenac sodium. Our findings demonstrate that Parangipattai kudineer chooranam drastically reduces free radicals responsible for inflammation and skin disorder in RAW cell line assay. However least viability of psoriatic cell was observed at the concentration of 250µg/ml and shows 88.62 ± 8.424 %, and posiblily destructing more than 80% of psoriatic cells in 250 µg/ml.

Conclusion: Thus concluding that the formulation mainly contains the herb Smilax china, i.e Parangipattai Kudineer Chooranam shows significant actions in Antiinflammatory properties, immunomodulatory action and anti-Psoriatic activity and may be due to presence of polyherbal components.

Author Biographies

Archana K

PG Scholar, Department of Sirappu Maruthuvam, National Institute of Siddha, Chennai, Tamil Nadu, India

Mahadevan MV

Lecturer, Department of Sirappu Maruthuvam, National Institute of Siddha, Chennai, Tamil Nadu, India.

Mahalakshmi V

Associate Professor, Department of Sirappu Maruthuvam, National Institute of Siddha, Chennai, Tamil Nadu, India.

Muthukumar NJ

Professor & Head of the Department, Department of Sirappu Maruthuvam, National Institute of Siddha, Chennai, Tamil Nadu, India.

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Published
2019-10-05
How to Cite
1.
Archana K, Mahadevan MV, Mahalakshmi V, Muthukumar NJ. In-vitro pharmacological screening of Parangipattai Kudineer chooranam - a herbal formulation used for Kalanjagapadai ( Psoriasis ). J Res Bio Med Sci [Internet]. 2019Oct.5 [cited 2025Oct.30];2(4):51-7. Available from: http://biosci.in/index.php/jrbms/article/view/42
Issue
Vol 2 No 4 (2019): Oct-Dec
Section
Articles

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